yqjD [2020-09-10 10:46:28]

propionyl-CoA carboxylase

locus: BSU_23920

pI: 9.09

mw: 55.12 kDa

protein length: 507 aa

gene length: 1524 bp

function: lipid metabolism

product: propionyl-CoA carboxylase

essential: no

ec: 6.4.1.3

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,486,813 → 2,488,336

The protein

Catalyzed reaction/ biological activity

• ATP + hydrogencarbonate + propanoyl-CoA --> (S)-methylmalonyl-CoA + ADP + H⁺ + phosphate (according to UniProt)

Protein family

• AccD/PCCB family (with [protein|E4997526CC534E38413030E90F7A135ECF65DF40|AccD] and [protein|2192474E13EAF6D2CF97CB957864FC89ED0118E0|YngE], according to UniProt)

Paralogous protein(s)

• [protein|2192474E13EAF6D2CF97CB957864FC89ED0118E0|YngE]

[SW|Domains]

• CoA carboxyltransferase N-terminal domain (aa 1-254) (according to UniProt)
• CoA carboxyltransferase C-terminal domain (aa 256-501) (according to UniProt)

Structure

• [PDB|1VRG] (from Thermotoga maritima, 57% identity)

Biological materials

Mutant

• MGNA-C387 (yqjD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2385 NBRP B. subtilis, Japan]
• BKE23920 (Δ[gene|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCGTGTAAAAATGATCCA, downstream forward: _UP4_CTTTAATATGGAGGGACTTA
• BKK23920 (Δ[gene|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCGTGTAAAAATGATCCA, downstream forward: _UP4_CTTTAATATGGAGGGACTTA